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Links to flybase: Argonaute 2
This graph displays the expression value in RPKM for each condition. RPKM: Reads Per Kilobase of transcript per Million.

I. Expression value (rpkm) per cell type

This graph displays the expression value in rpkm for each cell type in the midgut. The expression values originate from RNA sequencing realized on FACS sorted cell populations from the midgut.
ISC: intestinal stem cell (delta-Gal4>UAS-GFP), EB: enteroblast (Su(H)-Gal4>UAS-GFP), EC: enterocyte (Myo1A-Gal4>UAS-GFP), EE: enteroendocrine cell (prosperov1-Gal4>UAS-GFP), VM: visceral muscle (how-Gal4>UAS-GFP)
ISC EB EC EE VM
Argonaute 2 6.286 7.076 5.399 2.731 4.046
Argonaute 2/rpl32 0.01 0.012 0.011 0.011 0.01

II. Region specific expression per cell type

Combined graphs

This section displays rpkm values for each region and all cell types. The second graph is only displayed when one gene is searched. All cell types and regions are displayed in one single graph for easy comparison.
R1 R2 R3 R4 R5
Argonaute 2 16.871 23.266 14.786 23.786 28.748
Argonaute 2/rpl32 0.009 0.011 0.007 0.012 0.015

Individual Cell types:

These graphs display the expression value in rpkm for each region in each cell type along the midgut. The expression values originate from RNA sequencing realized on FACS sorted cell populations from dissected regions of the midgut.
ISC: intestinal stem cell (delta-Gal4>UAS-GFP), EB: enteroblast (Su(H)-Gal4>UAS-GFP), EC: enterocyte (Myo1A-Gal4>UAS-GFP), EE: enteroendocrine cell (prosperov1-Gal4>UAS-GFP), VM: visceral muscle (how-Gal4>UAS-GFP)
Regions are ordered from anterior (R1) to posterior (R5) and described elsewhere (Buchon et al, 2013, Cell reports).
R1 R2 R3 R4 R5
Argonaute 2 rpkm 3.411 8.184 3.662 7.645 8.527
Argonaute 2/rpl32 0.008 0.012 0.006 0.012 0.014
R1 R2 R3 R4 R5
Argonaute 2 6.295 7.443 5.776 6.886 8.979
Argonaute 2/rpl32 0.01 0.013 0.008 0.013 0.015
R1 R2 R3 R4 R5
Argonaute 2 5.73 5.457 3.225 6.079 6.505
Argonaute 2/rpl32 0.012 0.01 0.007 0.01 0.013
R1 R2 R3 R4 R5
Argonaute 2 1.435 2.182 2.123 3.176 4.737
Argonaute 2/rpl32 0.005 0.01 0.007 0.014 0.026
R1 R2 R3 R4 R5
Argonaute 2 4.412 4.257 1.268 6.229 4.061
Argonaute 2/rpl32 0.009 0.012 0.004 0.014 0.014

III. Fold induction after Pe infection

This graph displays the fold enrichment for each gene in each cell type in response to infection with the entomopathogenic bacteria Pseudomonas entomophila (Pe). The values originate from RNA sequencing realized on FACS sorted cell populations from whole midguts either unchallenged or infected with Pe. Flies were starved for 2 hrs and then infected for 48hrs with P.entomophila bacteria on filter paper. Cell populations were then isolated using FACS (based on size and cell type specific marker expression).
non-blue histograms: the value for those columns does not represent actual fold induction.
Red histogram: the gene is either switched on or its gene expression upon infection is multiple log higher than in basal condition
Green histogram: the gene is either switched off or its expression upon infection is multiple log lower than in basal condition.
ISC EB EC EE
Argonaute 2 -1.7 -2.8 7.1 3.5