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Links to flybase: unpaired 3
This graph displays the expression value in RPKM for each condition. RPKM: Reads Per Kilobase of transcript per Million.

I. Expression value (rpkm) per cell type

This graph displays the expression value in rpkm for each cell type in the midgut. The expression values originate from RNA sequencing realized on FACS sorted cell populations from the midgut.
ISC: intestinal stem cell (delta-Gal4>UAS-GFP), EB: enteroblast (Su(H)-Gal4>UAS-GFP), EC: enterocyte (Myo1A-Gal4>UAS-GFP), EE: enteroendocrine cell (prosperov1-Gal4>UAS-GFP), VM: visceral muscle (how-Gal4>UAS-GFP)
ISC EB EC EE VM
unpaired 3 35.054 97.962 57.658 8.285 41.604
unpaired 3/rpl32 0.058 0.162 0.112 0.034 0.107

II. Region specific expression per cell type

Combined graphs

This section displays rpkm values for each region and all cell types. The second graph is only displayed when one gene is searched. All cell types and regions are displayed in one single graph for easy comparison.
R1 R2 R3 R4 R5
unpaired 3 156.845 125.591 230.817 172.858 308.685
unpaired 3/rpl32 0.086 0.062 0.111 0.087 0.164

Individual Cell types:

These graphs display the expression value in rpkm for each region in each cell type along the midgut. The expression values originate from RNA sequencing realized on FACS sorted cell populations from dissected regions of the midgut.
ISC: intestinal stem cell (delta-Gal4>UAS-GFP), EB: enteroblast (Su(H)-Gal4>UAS-GFP), EC: enterocyte (Myo1A-Gal4>UAS-GFP), EE: enteroendocrine cell (prosperov1-Gal4>UAS-GFP), VM: visceral muscle (how-Gal4>UAS-GFP)
Regions are ordered from anterior (R1) to posterior (R5) and described elsewhere (Buchon et al, 2013, Cell reports).
R1 R2 R3 R4 R5
unpaired 3 rpkm 28.865 18.232 54.746 18.638 54.791
unpaired 3/rpl32 0.065 0.026 0.087 0.029 0.092
R1 R2 R3 R4 R5
unpaired 3 82.853 71.258 108.893 82.196 144.608
unpaired 3/rpl32 0.132 0.126 0.155 0.159 0.239
R1 R2 R3 R4 R5
unpaired 3 43.21 31.053 55.83 64.989 93.21
unpaired 3/rpl32 0.091 0.056 0.128 0.109 0.186
R1 R2 R3 R4 R5
unpaired 3 1.917 5.048 11.348 7.034 16.077
unpaired 3/rpl32 0.007 0.023 0.038 0.03 0.087
R1 R2 R3 R4 R5
unpaired 3 24.001 58.705 4.702 67.872 52.74
unpaired 3/rpl32 0.048 0.164 0.014 0.151 0.177

III. Fold induction after Pe infection

This graph displays the fold enrichment for each gene in each cell type in response to infection with the entomopathogenic bacteria Pseudomonas entomophila (Pe). The values originate from RNA sequencing realized on FACS sorted cell populations from whole midguts either unchallenged or infected with Pe. Flies were starved for 2 hrs and then infected for 48hrs with P.entomophila bacteria on filter paper. Cell populations were then isolated using FACS (based on size and cell type specific marker expression).
non-blue histograms: the value for those columns does not represent actual fold induction.
Red histogram: the gene is either switched on or its gene expression upon infection is multiple log higher than in basal condition
Green histogram: the gene is either switched off or its expression upon infection is multiple log lower than in basal condition.
ISC EB EC EE
unpaired 3 10.3 1.5 2.5 3.1